Detection flux | 96 |
Applicable supplies | 0.2ml 96-well plate, 8 tubes, single tube (transparent, frosted, milky white are applicable) |
Number of fluorescence channels | 6 |
Applicable fluorescein | Channel 1: FAM, SYBR Green I, SYTO 9, EvaGreen, LC Green Channel 2: HEX, VIC, TET, JOE Channel 3: ROX, Texas Red Channel 4: Cy5 Channel 5: Alexa Fluor 680 Channel 6: FRET |
Applicable probe | Taqman probe, molecular beacon probe, scorpion probe, FRET probe, etc. |
Reaction system | 0 ～ 100μl |
Linear range | 1 ～ 1010 copies |
Sample detection repeatability | Ct value CV≤0.5% |
Sample linear | /r/≥0.999 |
Control method | Stand-alone operation: Use the instrument's 10.4-inch touch screen and software system to create a new experiment and run it. Network operation: (1) Direct PC connection: After the instrument is connected to the PC via a point-to-point network, the application software on the computer is used to implement experiments, monitoring, data analysis and other operations; (2) LAN access: By setting the network parameters of the instrument, the instrument can be connected to the local LAN, so that any computer in the local area network can perform operations such as instrument monitoring, data synchronization, and analysis. |
Automatic sample bin | Sample compartment can be automatically popped / closed by touch screen control; When the pop-up state is closed, you can touch the sample compartment to close automatically. |
Power failure protection | With the function of automatically resuming the experiment when the power is cut off and then power on, there is no need to wait for the PC and software to open |
Data transmission | Experimental data can be imported and exported via USB flash drive |
Storage | Can store more than 1000 experiment data files |
Optical system |
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Light source | Bright long-life maintenance-free LED light source |
Detector | Photodiode (PD) |
Detection location | Top excitation, top scan |
Detection method | Six fluorescence channels are scanned from hole to hole simultaneously without fluorescence edge effect |
Detection time | Complete detection of 96 wells in 6 fluorescence channels in 7 seconds |
Excitation light wavelength | Channel 1: 465nm Channel 2: 527nm Channel 3: 580nm Channel 4: 632nm Channel 5: 680nm Channel 6: 465nm |
Detection light wavelength | Channel 1: 510nm Channel 2: 563nm Channel 3: 616nm Channel 4: 664nm Channel 5: 730nm Channel 6: 616nm |
Fluorescence linear | r≥0.990 |
Dynamic range for fluorescence detection | Dynamic range of fluorescence detection can be adjusted according to reagents |
Temperature Control System |
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Cover temperature | Room temperature ～ 110 ℃ |
Constant pressure hot cap | Compatible with consumables of different heights |
Module temperature | 0℃～100℃ |
Module temperature control principle | Peltier effect, semiconductor refrigeration technology |
Maximum temperature rise / fall rate | Maximum heating rate ≥6.1 ℃ / s Maximum cooling rate ≥5.0 ℃ / s |
Temperature uniformity | ±0.1℃ |
Temperature accuracy | ≤±0.1℃ |
Gradient temperature width | 1℃～40℃ |
Gradient temperature number | 12 columns |
Special temperature setting function | Supports thermal gradient PCR experiments, Long PCR, Touch Down PCR |
Software functions |
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Analysis function | Qualitative analysis, absolute quantitative analysis, relative quantitative analysis, endpoint fluorescence analysis, melting curve analysis, SNP analysis, etc. |
Report customization | Pre-stored multiple industry experiment report models; Board full open universal report function, users can customize report content and form; |
Rights management function | The administrator account can limit the functions of “manually set thresholds”, “running settings”, “running experiments”, and “analysis data” of ordinary accounts to manage the use rights of instruments of ordinary accounts |
Resource sharing function | After the instrument is connected to the PC, both parties can realize the synchronous sharing of experimental data through operations such as uploading and downloading. |
